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齊一生物科技(上海)有限公司>>ELISA試劑盒>>酶聯(lián)免疫分析試劑盒>>齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒

齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒

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更新時間:2018-09-26 16:59:28瀏覽次數(shù):98

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齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒英文名稱Human Mucin-5 subtype AC,MUC5AC ELISA Kit酶聯(lián)免疫夾心法用于檢測標本:血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等.產(chǎn)品的用途:僅供科研究課題使用!

詳細介紹

齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒英文名稱Human Mucin-5 subtype AC,MUC5AC ELISA Kit酶聯(lián)免疫夾心法用于檢測標本:血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等.產(chǎn)品的用途:僅供科研究課題使用!

齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存  過程 中  如有沉淀形成,應再次離心。
2.血漿:應根據(jù)標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養(yǎng)上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細收集上清。  檢測細胞內(nèi)的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩吮救诨笕匀槐3?-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求: 
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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