詳細介紹
齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒英文名稱Human Mucin-5 subtype AC,MUC5AC ELISA Kit酶聯(lián)免疫夾心法用于檢測標本:血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等.產(chǎn)品的用途:僅供科研究課題使用!
齊一生物*轉(zhuǎn)氨酶谷丙轉(zhuǎn)氨酶(ALTGPT)檢測試劑盒標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應再次離心。
2.血漿:應根據(jù)標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養(yǎng)上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細收集上清。 檢測細胞內(nèi)的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩吮救诨笕匀槐3?-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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9238240 BioRobot M48 User Manual
9238225 Sheath Delivery Tubing w. Connectors
9238158 Heat Transfer Adapter, microplate FB
9240660 Block, Plastic, QC, AP96/196
9240651 Internal BCR, Keyence, BL-180, AP96/196
9240626 Fuse, 8amp, 5 X 20 mm, AP196/98
9240623 Circuit Breaker, 2A, AP196/98
9240591 3-Way Mac Valve, AP196/96
9240574 Micro-Lynx w\speedbaord, AP98
9240572 Rotor w/Buckets, Sorvall, AP96
9240552 Freq. Conv., PCB, Eppendorf, AP196/98
9240531 Cable, Motor, Axis #7, Pump U/D, AP98
9240564 Fan, Condenser, 230V,Eppendorf, AP196/98
9240553 Cable, PC-104 to Eppendorf, AP196/98
9240548 Motor Cap Repair, Eppendorf, AP196/98
9240515 I/O PCB, Main Board, AP196/98
9240503 PM Kit, AP196
9239958 BR M48, PM Service "Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
9239853 Foot, complete, v2.0 (4)
9239572 BR Universal, Full Agreement The Full Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9239563 Assy,Kit,Retrofit,HoriCompressorMnt
9020023 L 26_7 Firmware, DML2
9019975 PCB, UV light assembly, Cxx
9238858 Basic Cover Agreement Plato7, 1yr The Basic Cover Agreement Plato7 provides full service coverage for the Plato 7 platform. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Basic Cover Agreement, the response time is 5 working days.
9241233 User Manual, QIAxcel FA 01/08 (WW)
9241227 ZLV, Handscanner, Qsym
9241180 Motor, Z, QX
9019473 Loading guide, 52 well sharkstooth, GSC
9019462 Switch, micro, HS
9019457 Fans (2), 5V, 30mm, HS
9237172 Cable USB
9233169 Board, YZ-CPU, BR8000
9237163 Bezel LiquiChip
9237162 Shipping Plug XYP
9237135 #Twister I, Full Agreement, no PM The Full Agreement BR Twister I provides full service coverage for the BioRobot Twister I. Costs for service visits are fully covered including travel, labor, and parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9237109 Protocol Dev./Opt., on-site (5-days) The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel and labor charges for a site visit up to 5 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, and accessories not included. Custom protocols are the sole responsibility of the customer and neither warrantied nor guaranteed.
9237102 Upgrade, BR plus 2 ext. devices Upgrade BioRobot with two approved integration devices
9232974 Card, x-main, peristaltic XPUPG
9232972 YZ 96 CPU Board/Driver Unit
9232947 Springcontact, RoboVac, BR8000
9236408 Storage Platform TW I, deep well
9232918 Board, PCON, auto vac, BR8000
9236334 Green LSR
9236324 Fan, LiquiChip
9236151 Locator Plate, 96 well,pos 2,BR RP
9230820 Rack for reagents, Universal (zig-zag)
9230718 Predilution-Rack 320x2ml Rose-80
9230365 Cable,Interface,flat,(Syr.)-Incub.-Read.
9021284 Safety lock, waste drawer, v1, Qsym
9238611 Rubber seal, centrifuge motor
9238477 Tubing, OLF pump head, RapidPlate
9238245 Tray for Reaction
9238191 Upgrade App., DirectPrep 96
9237721 EZ1, Full Agreement The Full Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9240676 Bimba Cylinder, Latch Lock, AP98
9240668 Nut, Flangeless,1/4-28, Blue, AP