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人E鈣粘著蛋白/上皮性鈣黏附蛋白(E-Cad)ELISA試劑盒英文名稱Human E-Cadherin,E-Cad ELISA試劑盒 齊一生物科技有限公司主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒,種類齊全,價(jià)格實(shí)惠,貨期短.購(gòu)買我公司產(chǎn)品新老客戶,可享全程技術(shù)指導(dǎo)：(包括售前標(biāo)本收集、后期實(shí)驗(yàn)、實(shí)驗(yàn)結(jié)束后數(shù)據(jù)分析等技術(shù)問題,我公司會(huì)有專業(yè)的技術(shù)即時(shí)給您去電溝通、解決.)
人E鈣粘著蛋白/上皮性鈣黏附蛋白(E-Cad)ELISA試劑盒操作步驟：
1、標(biāo)準(zhǔn)品的稀釋：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔，在*、第二孔中分別加標(biāo)準(zhǔn)品100μl，然后在*、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl，混勻；然后從*孔、第二孔中各取100μl分別加到第三孔和第四孔，再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻；然后在第三孔和第四孔中先各取50μl棄掉，再各取50μl分別加到第五、第六孔中，再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul，混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中，再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻后從第七、第八孔中分別取50μl加到第九、第十孔中，再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl，混勻后從第九第十孔中各取50μl棄掉。（稀釋后各孔加樣量都為50μl，濃度分別為9mmol/L，6 mmol/L，3 mmol/L，1.5mmol/L， 0.75 mmol/L）。
2、加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）、待測(cè)樣品孔。在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl，然后再加待測(cè)樣品10μl（樣品zui終稀釋度為5倍）。加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻。
3、溫育：用封板膜封板后置37℃溫育30分鐘。
4、配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用。
5、洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置30秒后棄去，如此重復(fù)5次，拍干。
6、加酶：每孔加入酶標(biāo)試劑50μl，空白孔除外。
7、溫育：操作同3。
8、洗滌：操作同5。
9、顯色：每孔先加入顯色劑A50μl，再加入顯色劑B50μl，輕輕震蕩混勻，37℃避光顯色15分鐘.
10、終止：每孔加終止液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。
11、測(cè)定：以空白空調(diào)零，450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）。 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。
26173 Sulfo-SDA (Sulfo-NHS-Diazirine) 50 mg
26174 Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) 50 mg
26175 Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) 50 mg
26178 Pierce Rapid Antibody Isotyping Kit - Mouse EA
26179 Pierce Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse EA
26180 Pierce HA-Tag IP/Co-IP Kit Kit
26181 HA Epitope Tag Antibody, Agarose conjugate (2-2.2.14) 1 mL
26182 HA Epitope Tag Antibody, Agarose conjugate (2-2.2.14) 5 mL RESIN
26183 HA Epitope Tag Antibody (2-2.2.14), 100 ug. 100 UG
人E鈣粘著蛋白/上皮性鈣黏附蛋白(E-Cad)ELISA試劑盒26184 Pierce HA Peptide 5 mg
26196 Pierce NHS-Activated Agarose, Dry 1 g
26197 Pierce NHS-Activated Agarose, Dry 5 G
26198 Pierce NHS-Activated Agarose Spin Columns, 0.2mL 25 SPIN COLU
26199 Pierce NHS-Activated Agarose Spin Columns, 2mL 5 SPIN COLUM
26200 Pierce NHS-Activated Agarose Slurry 25 mL.
26777 Sulfo-NHS-Acetate 100 mg
26922 PITC (Edman's Reagent) 10/1 mL
27712 Sulfo-SHPP (Water-Soluble Bolton-Hunter Reagent) 100 mg
27852 RBS 35 Concentrate 20 kg
27950 RBS 35 Concentrate 1 kg
27952 RBS 35 Concentrate 5 KGS
27954 RBS-35 CONCENTRATE, 220 KG 220 KG
27959 RBS pF Detergent Concentrate 1 kg
27960 RBS pF Detergent Concentrate 5 KG
27968 RBS Solid Detergent 5 KG
28005 Pierce Biotin Quantitation Kit Kit
28010 HABA 10 GM PKG.
28020 EZ-Link Hydrazide-Biocytin 25 mg
28022 EZ-Link Biocytin 100 mg
28100 PMPI 50 mg
28230 Tween-80 Surfact-Amps Detergent Solution 500 mL
28299 CHAPS 100 GM
28300 CHAPS 5 GM
28310 Octyl-beta-Glucoside 5 GM
28312 Sodium Dodecyl Sulfate (SDS), C12 500 GM
28313 Triton X-100 Surfact-Amps Detergent Solution 1000 mL
28314 Triton X-100 Surfact-Amps Detergent Solution 6/10 mL
28316 Brij-35 Surfact-Amps Detergent Solution 6/10 mL AMPS
28320 Tween-20 Surfact-Amps Detergent Solution 6/10 mL
28321 Tween-20 Surfact-Amps Detergent Solution 1000 mL
28324 NP-40 Surfact-Amps Detergent Solution 6 X 10 mL
28328 Tween-80 Surfact-Amps Detergent Solution 6 X 10 mL
28329 Tween-80 Surfact-Amps Detergent Solution 50 mL
28332 Triton X-114 Surfact-Amps Detergent Solution 6 X 10 mL
28336 Brij-58 Surfact-Amps Detergent Solution 6 X 10 mL
28340 Surfact-Amps Detergent Sampler Kit
28341 20X Borate Buffer 0.5 L
28344 20X Modified Dulbecco's PBS Buffer 0.5 mL
28346 20X Modified Dulbecco's PBS Tween-20 Buffer 0.5 L
Human soluble vascular endothelial growth factor receptor-2,VEGFR-2sFLK-1 ELISA Kit
Human thymic stromal lymphopoietin,TSLP ELISA Kit
Human PerforinPore-forming protein,PFPFP ELISA Kit
Human pleiotrophin,PTN ELISA Kit
human soluble cluster of differentiation 28,sCD28 ELISA Kit
Human lymphocyte factor ELISA Kit
Human thymus activation regulated chemokine,TARC ELISA Kit
Human Neural cell adhesion molecule ligand 1,NCAM-L1 ELISA Kit
Human Cobra venom neuronal protective factor,CVNPF ELISA Kit
Human soluble Tumor Necrosis Factorαreceptor,sTNFαR ELISA Kit
Human soluble cytokine receptor,sCKR ELISA Kit
Human soluble Factor-related Apoptosis ligand,sFASLApo-1 ELISA Kit
Human inhibitor of apoptosis,IAP ELISA Kit
Human colony-stimulating factor,CSF ELISA Kit
Human monocyte interferon gamma inducing factor,MIGF ELISA Kit
Human Interferon inducible T-cell Chemoattractant,I-TAC ELISA Kit
Human cluster Of differentiation,CDl4 ELISA Kit
Human apoptosis inducing factor,AIF ELISA Kit
Human leukocyte common antigen,LCACD45 ELISA Kit
Human cluster Of differentiation,CD4 ELISA Kit
Human Placenta Cadherin,P-cad ELISA Kit
Human Keratinocyte Growth Factor,KGF ELISA Kit
Human Plaet-Derived Growth Factor-BB,PDGF-BB ELISA kit
Human CXC-chemokine ligand 16,CXCL16 ELISA Kit
Human CXC-chemokine receptor 3,CXCR3 ELISA Kit
Human interferon-inducible protein 16,IFI16p16 ELISA Kit
Human Stromal cell derived factor 1a,SDF-1a ELISA Kit
Human lymphotactin,LptnLTN ELISA Kit
Human soluble Cluster of differentiation 86,sCD86 ELISA Kit
human Interleukin 27,IL-27 ELISA Kit
Human Macrophage Migration Inhibitory Factor,MIF ELISA Kit
Human interferon-inducible protein 10,IP-10 ELISA Kit
Human Interleukin 17,IL-17 ELISA Kit
Human Interleukin 1β,IL-1β ELISA Kit
人E鈣粘著蛋白/上皮性鈣黏附蛋白(E-Cad)ELISA試劑盒Human macrophage inflammatory protein 5,MIP-5 ELISA Kit
Human Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit
Human intercellular adhesion molecule 2,ICAM-2 ELISA Kit
Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit
Human connective tissue growth factor,CTGF ELISA Kit
Human Interleukin 18,IL-18 ELISA KIT
Human mucosae associated epithelia chemokine,MEC ELISA Kit
Human mucosae associated epithelia chemokine,MEC ELISA Kit
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